Search the EPA Research Database
Project Search Result
Project Code [TARGET-TB]
This information is correct as of today and is updated from time to time by the EPA to reflect changes in the management of the project. Please check back regularly for updates.
Project title
Targeted genome editing to enhance genetic resistance to Mycobacterium bovis infection in domestic cattle populations
Primary Funding Agency
Department of Agriculture, Food & the Marine
Co-Funding Organisation(s)
USDA -NIFA, DAERA
Lead Organisation
University College Dublin (UCD)
Project Abstract
The effective and early detection of disease within cattle is widely recognized as a critical component in maximizing clinical and therapeutic outcomes, increasing production efficiency and limiting the economic impact of infections. AgriSense II will develop an electronic sensor platform consists of two sensors with different sensing mechanisms (potentiometric sensors based on field-effect-transistors and electrochemical impedance) which can be co-fabricated on the same substrate that will greatly reduce false positive and false negative test results therefore providing more robust clinical data to stakeholder such as farmers and veterinarians. The device will target multiplexed (simultaneous) detection of commonly occurring viral pathogens known to cause production diseases that have high animal welfare and economic costs. These diseases include: Bovine Viral Diarrhoea (BVDV), Bovine Parainfluenza Virus-3 (BPIV-3), Bovine Respiratory Syncytial Virus (BRSV), and Enzootic Bovine Leucosis (EBL). New disease specific capture proteins to be developed by QUB will have high specificity and avidity for the selected disease will, for the first time, enable highly selective immunoassays. Electronic sensors will permit rapid diagnostics (in less than 10 minutes) while the dual sensing approach will provide results with higher confidence levels than are currently available due to reduce false positives (negatives). The sensor device will also consist of positive and negative control sensor channels to (i) confirm assay functionality and (ii) correct for non-specific bind, noise and drift. Finally the devices will be validated in a commercial veterinary diagnostics laboratory using archived samples diseases whose control and prevention are critical to the cattle and dairy industries in the United States, the Republic of Ireland and Northern Ireland. This will be followed by an extensive on-farm diagnostic validation using milk samples followed by demonstration activities to various stakeholders on-farm.
Grant Approved
�325,115.00
Research Theme
Ireland's Future Climate, its Impacts, and Adaptation Options
Initial Projected Completion Date
31/05/2021