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Project Code [2011-EH-MS-11]
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Project title
CapE -Capture, Extract, Amplify: A Rapid Method for Monitoring Large Water Volumes for Pathogenic Contaminants?
Primary Funding Agency
Environmental Protection Agency
Co-Funding Organisation(s)
n/a
Lead Organisation
National University of Ireland Galway (NUIG)
Lead Applicant
Dearbhaile Morris
Project Abstract
Ireland has one of the highest rates of infection with verotoxigenic E. coli (VTEC) in the European Union. VTEC are commonly present in the gastrointestinal tract of cattle and Ireland has a very large bovine population many of which are grass fed outdoors and shedding faeces into the general environment for much of the year. Ireland has a very high level of rainfall which contributes to the risk of run-off of surface deposited animal faeces into surface and ground waters. There is a consensus that contaminated water is a significant contributor to VTEC infection in Ireland and elsewhere. Existing conventional methods for detection of VTEC have significant limitations because of (1) the complexity of VTEC biology and (2) low level and intermittent contamination of water may be associated with disease given the very low infectious dose. This project proposes to develop an approach to sampling of large volumes of water for VTEC using molecular methods capable of detecting essentially all biological variants of VTEC. The approach proposed, if successful, could be readily adapted, using other molecular targets to detect other E. coli variants of public health concern as well as a wider range of other water-borne bacteria and protozoan pathogens In terms of academic output, this project will result in at a number of peer reviewed papers, and several conference presentations. This research will increase capacity and strengthen links between the institutions involved, and inform policy related to the water quality and its management. Recommendations to local enforcement and/or regulatory authorities on potential monitoring strategies will be provided
Grant Approved
�249,994.77
Research Hub
Healthy Environment
Research Theme
Environment and Human Health
Initial Projected Completion Date
03/03/2014