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Project Code [2007-FS-WRM-5-M5]
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Project title
Development of specific bacterial detoxification enzymes as bioindicators and biosensors of environmental pollution
Primary Funding Agency
Environmental Protection Agency
Co-Funding Organisation(s)
n/a
Lead Organisation
Carlow Institute of Technology (IT Carlow)
Lead Applicant
David Dowling
Project Abstract
Toxic chemicals introduced into the environment, via industry or agriculture, can penetrate ecosystems and pose a threat to all species from bacteria to humans. Therefore, interest in the use of bioindicators and biosensors in environmental monitoring is growing rapidly. In this proposal, we use molecular biological technologies to detect genes (known and novel) for bacterial detoxification enzymes [glutathione S-transferases (GSTs)] in soil or sediment samples from selected polluted sites in Ireland. Bacterial GST profiles and microbial community profiles associated with these sites will be evaluated for their potential as bioindicators of environmental toxicity. Finally, a specific bacterial GST, BphKLB400, will be developed as a biosensor to be used in environmental monitoring of toxic chlorinated organic compounds.
Grant Approved
�237,355.20
Research Hub
Green and Circular Economy
Research Theme
Waste and Resources Management
Initial Projected Completion Date
01/08/2010