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Project Code [2007-FS-WRM-5-M5]

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Project title

Development of specific bacterial detoxification enzymes as bioindicators and biosensors of environmental pollution

Primary Funding Agency

Environmental Protection Agency

Co-Funding Organisation(s)

n/a

Lead Organisation

Carlow Institute of Technology (IT Carlow)

Lead Applicant

David Dowling

Project Abstract

Toxic chemicals introduced into the environment, via industry or agriculture, can penetrate ecosystems and pose a threat to all species from bacteria to humans. Therefore, interest in the use of bioindicators and biosensors in environmental monitoring is growing rapidly. In this proposal, we use molecular biological technologies to detect genes (known and novel) for bacterial detoxification enzymes [glutathione S-transferases (GSTs)] in soil or sediment samples from selected polluted sites in Ireland. Bacterial GST profiles and microbial community profiles associated with these sites will be evaluated for their potential as bioindicators of environmental toxicity. Finally, a specific bacterial GST, BphKLB400, will be developed as a biosensor to be used in environmental monitoring of toxic chlorinated organic compounds.

Grant Approved

�237,355.20

Research Hub

Green and Circular Economy

Research Theme

Waste and Resources Management

Start Date

01/03/2008

Initial Projected Completion Date

01/08/2010