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Project Code [2007-PhD-ET-9]

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Project title

Exploitation of sugar kinases in green chemistry

Primary Funding Agency

Environmental Protection Agency

Co-Funding Organisation(s)

n/a

Lead Organisation

Queen's University Belfast (QUB)

Lead Applicant

David J Timson

Project Abstract

It is proposed to assess the potential of two related recombinant human enzymes ? galactokinase and N-acetylgalactosamine kinase ? as catalysts for low environmental impact site- and stereospecific phosphorylation of sugars.This will be achieved by1. Rational, structure-based mutagenesis of both enzymes to alter the substrate specificity thus providing new synthetic routes to sugar and N-acetyl sugar phosphates.2. Determining the effects of temperature, organic solvents and covalent immobilisation to a solid substrate (such as agarose or sepharose) on the enzymes to assess their suitability for use in scaled-up industrial processes.

Grant Approved

�95,000.00

Research Hub

Green and Circular Economy

Research Theme

Waste and Resources Management

Start Date

01/10/2007

Initial Projected Completion Date

01/10/2010